顯微光譜系統主要技術指標有哪些
更新時間:2020-1-6 0:00:00
在顯微光譜系統的相關技術當中,成像光譜技術和顯微技術是相結合起來的,兩者相互綜合而發揮出更大的優越性從而組成了顯微光譜系統,為了能夠保證該系統能夠達到預先使用的效果和目的,所以需要對其技術指標有所了解,只有達到以下幾個技術指標的產品才能夠達到質量要求。
一、光譜范圍
顯微角分辨光譜公司介紹因為一般來說光譜范圍需要能夠包括所處理的樣品的特性,并且還應該根據分工方式來確定。為了所選用的顯微光譜系統可以相關物體的光伏情況,所以有必要對光譜范圍做一定的要求,并且還可根據反射光吸收光譜范圍以及所激發的熒光光譜范圍綜合確定工作光譜范圍。
二、譜段數
在選取顯微光譜系統的譜段數的時候,需要先弄清楚CCD面陣相機的光譜像元數,所以是沒有特定標準的。不過對某些品牌的顯微光譜系統來說,也可以根據以往的經驗來選擇合理的范圍,比如有些操作者會選擇譜段數為2的整數次冪等等。
三、光譜分辨率
顯微光譜系統的光譜分辨率是比較重要的一個參數和技術指標,所以應該要對此了解清楚,并且它主要和分工系統的設計有著不可分割的關系,而且如果是色散型的風光系統那么,它還跟兩者之間的夾縫寬度有關系。所以要從這些方面出發去進行辨別顯微光譜系統的技術情況,其中狹縫的寬窄和光譜分辨率成反比,并且同時還應該要考慮到實際應用的問題。
以上就是想為光譜系統的主要技術指標所包括的幾個方面,另外,還有空間分辨率也是其中的參數之一。這主要由顯微物鏡的數值孔徑以及放大倍率和CCD像元大小而定,并且在采用激光掃描共焦熒光顯微光譜系統中,這種設備比普通顯微系統的空間分辨率提高很多。
Among the related technologies of microspectral system, imaging spectrum technology and microspectral technology are combined. They combine with each other to give full play to their greater advantages and thus constitute a microspectral system. In order to ensure that the system can achieve the effect and purpose of pre-use, it is necessary to understand its technical indicators. Only products that achieve the following technical indicators are needed To meet the quality requirements.
1、 Spectral range
In general, the spectrum range needs to be able to include the characteristics of the processed samples, and should also be determined according to the division of labor. In order to select the microspectral system that can be related to the photovoltaic situation of the object, it is necessary to make certain requirements for the spectral range, and also can determine the working spectral range comprehensively according to the absorption spectral range of reflected light and the excited fluorescence spectral range.
2、 Number of spectral segments
When selecting the number of spectral segments of the microspectral system, we need to know the number of spectral pixels of CCD array camera first, so there is no specific standard. However, for some brands of microspectral systems, we can also choose a reasonable range based on previous experience, for example, some operators will choose the integral power of the number of spectral segments as 2, and so on.
3、 Spectral resolution
The spectral resolution of microspectral system is an important parameter and technical index, so it should be understood clearly, and it mainly has an inseparable relationship with the design of the division system, and if it is a dispersive system, it also has a relationship with the gap width between them. Therefore, it is necessary to identify the microspectral system from these aspects, in which the width of the slit is inversely proportional to the spectral resolution, and at the same time, the practical application should be considered.
The above are the main technical specifications of the spectral system, in addition, the spatial resolution is also one of the parameters. This is mainly determined by the numerical aperture, magnification and CCD pixel size of the microscope objective. In the laser scanning confocal fluorescence microscope system, the spatial resolution of this equipment is much higher than that of the ordinary microscope system.